Capillary isotachophoresis-mass spectrometric determination of DNA damage
Conference
·
OSTI ID:210633
- Pacific Northwest Lab., Richland, WA (United States)
Analysis of DNA damage as indicators of human exposure to radiation levels represents a challenge for trace analyses to analytical chemists. Mutagenesis is one possible outcome of DNA base modification arising from such damage. These modifications may lead to DNA misreplication which can initiate and promote carcinogenesis. Electrospray ionization mass spectrometry (ESI-MS) with high ionization efficiency and gentle nature can be used to identify and quantify these modifications and opens the opportunity to study structurally related factors in the damage to DNA. The radiation-damaged products are generally present in very low abundance, which requires a highly efficient and/or concentrating separation technique prior to mass spectrometric detection. High performance capillary isotachophoresis (CITP), with its zone self-sharping and concentrating capacities, can be utilized to distinguish the small amount damaged from the large excesses of native free bases, nucleosides, and nucleotides. In combination with electrospray ionization, the potential exits to obtain extraordinary sensitivity.
- OSTI ID:
- 210633
- Report Number(s):
- CONF-9505261--
- Country of Publication:
- United States
- Language:
- English
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