CITP ESI-FTICR for the study of X-irradiation damaged tetranucleotides

Conference · Sun Dec 31 04:00:00 EST 1995

OSTI ID:210624

Hofstadler, S A; Zhao, Zhongxi; Smith, R D ^[1]

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1. Pacific Northwest Lab., Richland, WA (United States); and others

A unique advantage of capillary isotachophoresis (CITP) is the ability to pre-concentrate low-level analytes and subsequently perform high resolution separations starting with relatively large (and dilute) sample volumes. The inherent isoelectric focusing and purification common to CITP make it well suited for trace analysis. The coupling of this technique to a high performance mass spectrometric detection scheme provides an unprecedented opportunity to obtain molecular weight and structural information from analytes which are present in a concentration limited regime. The authors have recently combined CITP with electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometry in order to analyze and identify low level DNA damage products which result from the X-irradiation of oligomeric DNA in N{sub 2}-saturated aqueous solutions. Damage to DNA is a critical event in mutagenesis, as well as for the initiation and possibly the promotion of carcinogenesis. The necessity of degrading polymeric DNA to the monomer level in order to carry out analyses has invariably complicated the study of DNA damage. Using short oligodeoxynucleotides of specific length and sequence, it is possible to isolate modified oligomers bearing specific lesions and to carry out analysis on the intact modified oligomers.

• OSTI ID: 210624
• Report Number(s): CONF-9505261--
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
AQUEOUS SOLUTIONS
CHEMICAL RADIATION EFFECTS
ISOTACHOPHORESIS
MASS SPECTROSCOPY
OLIGONUCLEOTIDES
X RADIATION