The design and performance of an In-field ESI-FTICR instrument

Conference · Sun Dec 31 04:00:00 EST 1995

OSTI ID:210720

Wu, Qinyuan; Udseth, H R; Anderson, G A ^[1]

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1. Pacific Northwest Lab., Richland, WA (United States); and others

The coupling of electrospray ionization (ESI) with Fourier Transform Ion Cyclotron Resonance mass spectrometry (FTICR-MS) has been shown to be a promising tool for biological research. Most of the current schemes of combining ESI with FTICR involve forming ions outside the magnet and injecting into the magnetic field through either radio frequency ion guides or electrostatic lenses. Substantial ion losses occur during ion transportation for such external source ESI-FTICR, offsetting the high sensitivity in detection. Alternatively, ESI can be performed inside the magnet bore to significantly increase ion current in the cell. However, such an in-field ESI scheme requires critical differential pumping and ion optics design to maintain ultra-high vacuum in the cell region in order to achieve resolution similar to that obtained from external source scheme. In our in-field ESI-FTICR instrument, a home-made ESI probe is inserted into a superconductor magnet (max. 4.7 T, but presently at 1 T) so that electrospray generated ions are confined by the magnetic field and are injected into the ICR cell located 20 cm from the ESI source.

• OSTI ID: 210720
• Report Number(s): CONF-9505261--
• Country of Publication: United States
• Language: English

Related Subjects

44 INSTRUMENTATION
INCLUDING NUCLEAR AND PARTICLE DETECTORS
DESIGN
ION CYCLOTRON RESONANCE SPECTROSCOPY
MASS SPECTROMETERS
MASS SPECTROSCOPY
PERFORMANCE