The design and performance of an In-field ESI-FTICR instrument
- Pacific Northwest Lab., Richland, WA (United States); and others
The coupling of electrospray ionization (ESI) with Fourier Transform Ion Cyclotron Resonance mass spectrometry (FTICR-MS) has been shown to be a promising tool for biological research. Most of the current schemes of combining ESI with FTICR involve forming ions outside the magnet and injecting into the magnetic field through either radio frequency ion guides or electrostatic lenses. Substantial ion losses occur during ion transportation for such external source ESI-FTICR, offsetting the high sensitivity in detection. Alternatively, ESI can be performed inside the magnet bore to significantly increase ion current in the cell. However, such an in-field ESI scheme requires critical differential pumping and ion optics design to maintain ultra-high vacuum in the cell region in order to achieve resolution similar to that obtained from external source scheme. In our in-field ESI-FTICR instrument, a home-made ESI probe is inserted into a superconductor magnet (max. 4.7 T, but presently at 1 T) so that electrospray generated ions are confined by the magnetic field and are injected into the ICR cell located 20 cm from the ESI source.
- OSTI ID:
- 210720
- Report Number(s):
- CONF-9505261-; TRN: 96:001157-0159
- Resource Relation:
- Conference: 43. American Society of Mass Spectrometry (ASMS) conference on mass spectrometry and allied topics, Atlanta, GA (United States), 21-26 May 1995; Other Information: PBD: 1995; Related Information: Is Part Of Proceedings of the 43rd ASMS conference on mass spectrometry and allied topics; PB: 1411 p.
- Country of Publication:
- United States
- Language:
- English
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