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Title: Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry

Abstract

A new electrospray ionization interface for capillary zone electrophoresis-mass spectrometry (CZE-MS) is described. The interface uses a sheath flow of liquid to make the electrical contact at the CZE terminus, thus defining both the CZE and electrospray field gradients. This allows the composition of the electrosprayed liquid to be controlled independently of the CZE buffer, providing operation with buffers that could not be used previously (e.g., aqueous and high ionic strength buffers). The interface operation is independent of CZE flow rate; CZE capillaries are easily replaced and require no additional preparation. Since the electrospray occurs directly from the CZE capillary terminus, additional mixing volumes and metal surfaces are avoided and electrophoretic separation efficiency appears unperturbed. The dead volume associated with the electrospray interface is < 10 nL, corresponding to < 0.1 s for typical flow rates of the sheath electrode liquid. CZE-MS separations for mixtures of quaternary phosphonium salts and for epinephrine and related amines are demonstrated. Operation is demonstrated with high surfactant concentrations, as required for capillary electrokinetic chromatography. The extension to other capillary electrophoresis methods, such as isotachophoresis and isoelectric focusing, appears feasible.

Authors:
; ;
Publication Date:
Research Org.:
Pacific Northwest Lab., Richland, WA (USA)
OSTI Identifier:
6723720
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Journal Article
Journal Name:
Anal. Chem.; (United States)
Additional Journal Information:
Journal Volume: 60:18
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; EQUIPMENT INTERFACES; DESIGN; CAPILLARIES; ELECTROPHORESIS; EXPERIMENTAL DATA; FLOW RATE; IONIZATION; MASS SPECTROSCOPY; QUANTITATIVE CHEMICAL ANALYSIS; TRACE AMOUNTS; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; CHEMICAL ANALYSIS; DATA; INFORMATION; NUMERICAL DATA; ORGANS; SPECTROSCOPY; 400105* - Separation Procedures; 400102 - Chemical & Spectral Procedures

Citation Formats

Smith, R D, Barinaga, C J, and Udseth, H R. Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry. United States: N. p., 1988. Web. doi:10.1021/ac00169a022.
Smith, R D, Barinaga, C J, & Udseth, H R. Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry. United States. https://doi.org/10.1021/ac00169a022
Smith, R D, Barinaga, C J, and Udseth, H R. 1988. "Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry". United States. https://doi.org/10.1021/ac00169a022.
@article{osti_6723720,
title = {Improved electrospray ionization interface for capillary zone electrophoresis-mass spectrometry},
author = {Smith, R D and Barinaga, C J and Udseth, H R},
abstractNote = {A new electrospray ionization interface for capillary zone electrophoresis-mass spectrometry (CZE-MS) is described. The interface uses a sheath flow of liquid to make the electrical contact at the CZE terminus, thus defining both the CZE and electrospray field gradients. This allows the composition of the electrosprayed liquid to be controlled independently of the CZE buffer, providing operation with buffers that could not be used previously (e.g., aqueous and high ionic strength buffers). The interface operation is independent of CZE flow rate; CZE capillaries are easily replaced and require no additional preparation. Since the electrospray occurs directly from the CZE capillary terminus, additional mixing volumes and metal surfaces are avoided and electrophoretic separation efficiency appears unperturbed. The dead volume associated with the electrospray interface is < 10 nL, corresponding to < 0.1 s for typical flow rates of the sheath electrode liquid. CZE-MS separations for mixtures of quaternary phosphonium salts and for epinephrine and related amines are demonstrated. Operation is demonstrated with high surfactant concentrations, as required for capillary electrokinetic chromatography. The extension to other capillary electrophoresis methods, such as isotachophoresis and isoelectric focusing, appears feasible.},
doi = {10.1021/ac00169a022},
url = {https://www.osti.gov/biblio/6723720}, journal = {Anal. Chem.; (United States)},
number = ,
volume = 60:18,
place = {United States},
year = {1988},
month = {9}
}