Analysis of changes in natural cytostatic and cytotoxic activity of mouse spleen cells after administration of cyclophosphamide and. cap alpha. -interferon

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

OSTI ID:6591498

Van'ko, L V; Bogdashin, I V; Sukhikh, G T; Totibadze, N V; Fuks, B B

The authors studied the effects of cyclophosphamide and alpha-interferon on the natural cytostatic and cytotoxic activity of mouse spleen cells on lymphoma cells. The cells were incubated in medium RPMI-1640 with 10% fetal serum and 1% glutamine in the presence of tritiated thymidine. Incorporated radioactivity was estimated by scintillation counting. The natural cytotoxic activity of the cells was determined by the release of chromium 51 from labelled lymphoma cells transplanted in vitro. Pretreatment of spleen cells with leukocytic interferon was found to cause an increase in both cytotoxic and cytostatic activity of these cells. An investigation of the time course of the antimitotic activity of the cells after pretreatment with cyclophosphamide, causing the death of proliferating cells, showed that effectors of natural cytotoxic activity constitute a homogeneous group as regards sensitivity to cyclophosphamide. Cells exerting cytostatic activity in vitro are evidently heterogeneous for sensitivity to cyclophosphamide. Thymus and bone marrow cells were used for comparative purposes.

• Research Organization: Institute of Human Morphology, Moscow, USSR
• OSTI ID: 6591498
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Journal Name: Bull. Exp. Biol. Med. (Engl. Transl.); (United States) Vol. 102:8; ISSN BEXBA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKYLATING AGENTS
ANIMAL CELLS
ANIMALS
ANTIMITOTIC DRUGS
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BONE MARROW CELLS
CELL KILLING
CELL PROLIFERATION
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOXAN
EVEN-ODD NUCLEI
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOSUPPRESSIVE DRUGS
INHIBITION
INTERFERON
INTERMEDIATE MASS NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LYMPHOKINES
LYMPHOMAS
MAMMALS
MICE
MITOGENS
NEOPLASMS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIMIDINES
RADIOASSAY
RADIOISOTOPES
RIBOSIDES
RODENTS
SOMATIC CELLS
SPLEEN CELLS
THYMIDINE
THYMUS CELLS
TIME DEPENDENCE
TOXICITY
TRANSPLANTS
TRITIUM COMPOUNDS
TUMOR CELLS
UPTAKE
URACILS
URIDINE
VERTEBRATES