Excretion and tissue disposition of dichloroacetonitrile in rats and mice
The excretion and tissue distribution of (1-/sup 14/C)dichloroacetonitrile and (2-/sup 14/C)dichloroacetonitrile were studied in male Fischer 344 rats and male B6C3F1 mice. Three dose levels of dichloroacetonitrile (DCAN) (0.2, 2, or 15 mg/kg) were administered to rats and two dose levels of DCAN (2 or 15 mg/kg) to mice. Daily excreta including exhaled volatiles and radiolabeled carbon dioxide (/sup 14/CO/sub 2/) were analyzed for radiolabeled carbon (/sup 14/C) until greater than 70% of the radioactivity was excreted. At that time the animals were sacrificed and tissues were collected. Tissues and excreta were analyzed for /sup 14/C by combustion and liquid scintillation counting. Rats administered (1-/sup 14/C)DCAN excreted 62 to 73% of the /sup 14/C in 6 days, with 42 to 45% in urine, 14 to 20% in feces, and 3 to 8% as CO/sub 2/. Rats administered (2-/sup 14/C)DCAN excreted 82 to 86% of the /sup 14/C in 48 hr, with 35 to 40% in urine, 33 to 34% as CO/sub 2/, and 10 to 13% in feces. Mice administered (1-/sup 14/C)DCAN excreted 83 to 85% of the /sup 14/C in 24 hr, with 64 to 70% in urine, 9 to 13% in feces, and 5 to 6% as CO/sub 2/. Mice administered (2-/sup 14/C)DCAN excreted 84 to 88% of the /sup 14/C in 24 hr with 42 to 43% in urine, 8 to 11% in feces, and 31 to 37% as CO/sub 2/. Liver tissues retained the most /sup 14/C in all studies except the study of (1-/sup 14/C)DCAN in rats, where blood contained the most /sup 14/C. These results indicate that DCAN was absorbed rapidly after oral administration in water. The differences in the route of excretion of (1-/sup 14/C)DCAN compared to (2-/sup 14/C)DCAN indicated that the molecule was being cleaved in the body and metabolized by different mechanisms.
- Research Organization:
- Univ. of Arizona, Tucson, AZ (United States)
- OSTI ID:
- 6583337
- Journal Information:
- Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States)
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ACETONITRILE
METABOLISM
TISSUE DISTRIBUTION
BIODEGRADATION
CARBON 14 COMPOUNDS
CARBON DIOXIDE
DISINFECTANTS
EXCRETION
MICE
RATS
SCINTILLATION COUNTING
TRACER TECHNIQUES
URINE
WATER SUPPLY
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
CHEMICAL REACTIONS
CLEARANCE
COUNTING TECHNIQUES
DECOMPOSITION
DISTRIBUTION
GERMICIDES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
NITRILES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
RODENTS
VERTEBRATES
WASTES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques