Environmental stress mediated changes in transcriptional and translational regulation of protein synthesis in crop plants. Progress report, August 1980-April 1981

Technical Report · Wed Apr 01 04:00:00 EST 1981

DOI:https://doi.org/10.2172/6583195· OSTI ID:6583195

Key, J L

The response of soybean seedling tissues to heat stress and to a lesser extent to other stresses including water, anaerobiosis, DNP, salt, and supraoptimal levels of 2,4-D was studied. A detailed analysis of polyribosome/monoribosome transitions was made in the case of heat shock, and salt and water stresses. Poly(A) RNAs isolated from stressed tissues were translated, and cDNA made to poly(A) RNA from heat shock tissue was cloned, and selected clones used to assess changes in the homologous mRNA(s) during stress induction/recovery. cDNAs were also used in hybridization analyses to assess the complexity and abundance distribution of poly(A) RNAs of control and heat shocked tissue. In vivo labeling studies were made to assess the pattern of protein synthesis during the induction of and recovery from heat stress. There was a rapid loss of polyribosomes following transfer of tissue from 28/sup 0/ to 40/sup 0/. During water stress and salt stress, there was a gradual and near complete loss of polyribosomes. Polyribosomes and poly(A) RNA isolated from those polyribosomes isolated from both 28/sup 0/ and 40/sup 0/ tissue were translated in in vitro systems. In both cases a normal spectrum of translation products was obtained at both 28/sup 0/ and 40/sup 0/; in addition, a number of new proteins was observed in the 40/sup 0/ system. Intact seedlings or slices of hypocotyls incubated at 40/sup 0/ demonstrated a new pattern of in vivo labeled proteins. Synthesis in vivo of normal (28/sup 0/) proteins is greatly inhibited at 40/sup 0/ to 42/sup 0/. The normal 28/sup 0/ in vivo pattern of protein synthesis is restored rapidly following transfer of 40/sup 0/ to 42/sup 0/ heat-shocked tissue to 28/sup 0/. (ERB)

• Research Organization: Georgia Univ., Athens, GA (USA). Dept. of Botany
• Sponsoring Organization: USDOE
• DOE Contract Number: AS09-80ER10678
• OSTI ID: 6583195
• Report Number(s): DOE/ER/10678-1
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
553000 -- Agriculture & Food Technology
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ALKALI METAL COMPOUNDS
ANAEROBIC CONDITIONS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL STRESS
BIOSYNTHESIS
BODY TEMPERATURE
CHLORIDES
CHLORINE COMPOUNDS
CULTIVATION TECHNIQUES
DNA
GLYCINE HISPIDA
HALIDES
HALOGEN COMPOUNDS
HERBICIDES
HYBRIDIZATION
HYPERTHERMIA
LEGUMINOSAE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PESTICIDES
PLANTS
POTASSIUM CHLORIDES
POTASSIUM COMPOUNDS
PROTEINS
RECOVERY
RNA
SYNTHESIS
TRANSCRIPTION