Heat shock proteins of higher plants

Journal Article · Mon Jun 01 04:00:00 EDT 1981 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.78.6.3526· OSTI ID:6195085

Key, J L ^[1]; Lin, C Y; Chen, Y M

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1. Univ. of Georgia, Athens

The pattern of protein synthesis changes rapidly and dramatically when the growth temperature of soybean seedling tissue is increased from 28/sup 0/C (normal) to about 40/sup 0/C (heat shock). The synthesis of normal proteins is greatly decreased and a new set of proteins, heat shock proteins, is induced. The heat shock proteins of soybean consist of 10 new bands on one-dimensional NaDodSO/sub 4/ gels; a more complex pattern is observed on two-dimensional gels. When the tissue is returned to 28/sup 0/C after 4 h at 40/sup 0/C, there is progressive decline in the synthesis of heat shock proteins and reappearance of a normal pattern of synthesis by 3 or 4 h. In vitro translation of poly(A)/sup +/RNAs isolated from tissues grown at 28 and 40/sup 0/C shows that the heat shock proteins are translated from a new set of mRNAs induced at 40/sup 0/C; furthermore, the abundant class mRNAs for many of the normal proteins persist even though they are translated weakly in vivo at 40 or 42.5/sup 0/C. The heat shock response in soybean appears similar to the much-studied heat shock phenomenon in Drosophila.

• OSTI ID: 6195085
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 78:6; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560203* -- Thermal Effects-- Plants-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ARTHROPODS
BIOCHEMISTRY
BIOLOGICAL STRESS
BIOSYNTHESIS
CHEMISTRY
COMPARATIVE EVALUATIONS
DIPTERA
DROSOPHILA
ENERGY
ENZYME ACTIVITY
FLIES
FRUIT FLIES
FUNCTIONS
GLYCINE HISPIDA
GROWTH
HEAT
IN VITRO
INSECTS
INVERTEBRATES
LEGUMINOSAE
MORPHOLOGICAL CHANGES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANT GROWTH
PLANTS
PROTEINS
RESPONSE FUNCTIONS
RNA
SEEDLINGS
SYNTHESIS
TEMPERATURE EFFECTS
THERMAL SHOCK
ULTRASTRUCTURAL CHANGES