An analysis of mRNAs for a group of heat shock proteins of soybean using cloned cDNAs
Journal Article
·
· J. Mol. Appl. Genet.; (United States)
OSTI ID:5405382
Hybridization studies carried out with poly(A)/sup +/ RNA and its corresponding cDNA showed the presence of a new highly abundant RNA class after heat shock (hs) at 40/sup 0/C in soybean hypocotyl compared to tissue incubated under normal conditions at 28/sup 0/C. cDNA clones complementary to RNAs of this class were isolated; eleven clones were characterized and used in the analysis of these abundant RNAs. The most abundant hs-sequences were found to be 800-900 nucleotides in length and present in about 19,000 copies per cell. Extensive sequence homology among hs-induced RNAs was indicated by cross-hybridization of cDNA clones and by common protein patterns generated in hybrid release translations. The existence of at least two different nucleotide sequences common to several different hs-poly(A)/sup +/ mRNAs was documented by different, nonoverlapping protein patterns obtained by in vitro synthesis with hybrid selected RNAs. Four clones contained a sequence common to mRNAs for a least 13 proteins of 15,000-18,000 daltons; another sequence common to mRNA for three to four proteins of 21,000-23,000 daltons was selected by one clone. Two other clones selected a major hs-protein of about 18,000 daltons. The mRNAs of these low molecular weight hs-proteins accumulated rapidly after induction at either 40/sup 0/C or 42.5/sup 0/C and decreased rapidly during subsequent incubation at 28/sup 0/C.
- DOE Contract Number:
- AS09-80ER10678
- OSTI ID:
- 5405382
- Journal Information:
- J. Mol. Appl. Genet.; (United States), Journal Name: J. Mol. Appl. Genet.; (United States) Vol. 1; ISSN JMAGD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CLONING
DNA-CLONING
GLYCINE HISPIDA
HEAT TREATMENTS
HYBRIDIZATION
IN VITRO
LEGUMINOSAE
MESSENGER-RNA
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PLANTS
PROTEIN DENATURATION
PROTEINS
RNA
TEMPERATURE DEPENDENCE
TEMPERATURE EFFECTS
59 BASIC BIOLOGICAL SCIENCES
CLONING
DNA-CLONING
GLYCINE HISPIDA
HEAT TREATMENTS
HYBRIDIZATION
IN VITRO
LEGUMINOSAE
MESSENGER-RNA
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PLANTS
PROTEIN DENATURATION
PROTEINS
RNA
TEMPERATURE DEPENDENCE
TEMPERATURE EFFECTS