Induction and accumulation of heat shock-specific poly(A sup + ) RNAs and proteins in soybean seedlings during arsenite and cadmium treatments

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.86.4.1048· OSTI ID:6945806

Edelman, L; Czarnecka, E; Key, J L ^[1]

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1. Univ. of Georgia, Athens (USA)

Northern blot hybridization analyzes revealed that poly(A{sup +}) RNAs homologous to eight heat shock (HS)-specific cDNA clones were induced by arsenite (As) or Cd treatments. The mRNAs accumulated slower, and maximum accumulations were consistently lower than HS-induced levels. Prolonged treatment with low concentrations (50-100 micromolar) of As for 6 hours, or Cd for 12 hours, resulted in decreased accumulations of HS-specific mRNAs. This response resembled the autoregulation observed during continuous 40{degree}C HS. However, no autoregulation was evident when soybean seedlings were exposed to high concentrations of As (250 micromolar) or Cd (1 millimolar) for 12 hours. The cDNA probe pCE54 detected a second higher molecular weight poly(A{sup +}) RNA following As or Cd treatments which accumulated concomitantly with the lower molecular weight HS-specific poly(A{sup +}) RNA. The patterns of low molecular weight HS polypeptides from in vitro translations induced by HS, As, and Cd, and analyzed by one-dimensional and two-dimensional SDS-PAGE, were similar but temporal differences were apparent. The chemical agents used in this study apparently induced the accumulation and translation of HS messages in vivo but not in the selective manner as observed during HS treatment.

• DOE Contract Number: AS09-80ER10678
• OSTI ID: 6945806
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 86:4; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ARSENIC
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
CADMIUM
CLONING
DNA HYBRIDIZATION
DNA-CLONING
ELEMENTS
GLYCINE HISPIDA
HEAT TREATMENTS
HYBRIDIZATION
IN VITRO
IN VIVO
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METALS
MOLECULAR WEIGHT
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
PLANTS
POLYPEPTIDES
PROTEINS
RNA
SEEDLINGS
SEMIMETALS
TRANSCRIPTION