Heat shock proteins of higher plants

Journal Article · Mon Jun 01 04:00:00 EDT 1981 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.78.6.3526· OSTI ID:6526820

Key, J L ^[1]; Lin, C Y; Chen, Y M

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1. Univ. of Georgia, Athens

The pattern of protein synthesis changes rapidly and dramatically when the growth temperture of soybean seedling tissue is increased from 28/sup 0/C (normal) to about 40/sup 0/C (heat shock). The synthesis of normal proteins is greatly decreased and a new set of proteins, heat shock proteins, is induced. The heat shock proteins of soybean consist of 10 new bands on one-dimensional NaDodSO/sub 4/ gels; a more complex pattern is observed on two-dimensional gels. when the tissue is returned to 28/sup 0/C after 4 hr at 40/sup 0/C, there is progressive decline in the synthesis of heat shock proteins and reappearance of a normal pattern of synthesis by 3 or 4 hr. In vitro translation of poly(A)/sup +/RNAs isolated from tissued grown at 28 and 40/sup 0/C shows that the heat shock proteins are translated from a ndw set of mRNAs induced at 40/sup 0/C; furthermore, the abundant class mRNAs for many of the normal proteins persist even though they are translated weakly (or not at all) in vivo at 40 or 42.5/sup 0/C. The heat shock response in soybean appears similar to the much-studied heat shock phenomenon in Drosophila.

• OSTI ID: 6526820
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 78:6; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560203* -- Thermal Effects-- Plants-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
ELECTROPHORESIS
FLUORESCENCE
GLYCINE HISPIDA
LABELLED COMPOUNDS
LEGUMINOSAE
LEUCINE
LUMINESCENCE
MESSENGER-RNA
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PLANTS
PROTEINS
RIBOSOMES
RNA
SEEDLINGS
SYNTHESIS
TEMPERATURE EFFECTS
THERMAL SHOCK
TRITIUM COMPOUNDS