Immunization against rodent malaria with cryopreserved irradiated sporozoites of Plasmodium berghei

Journal Article · Thu May 01 04:00:00 EDT 1980 · Am. J. Trop. Med. Hyg.; (United States)

OSTI ID:6566478

Orjih, A U; Nussenzweig, R S

The preparation and storage of Plasmodium berghai sporozoites for immunization purposes is described. The sporozoites were irradiated and frozen either at -75/sup 0/C or in liquid nitrogen. After various periods sporozoites were thawed and injected into A/J mice. At the end of the immunization period the animals were challenged with infective sporozoites of P. berghei and monitored for parasitemia. It was found that the storage did not appreciably alter the ability of the irradiated sporozoites to induce protective immunity in the recipient animals. The highest protection (80 to 100%) has induced with sporozoites maintained in 10% serum and stored at -75/sup 0/C.

• Research Organization: New York Univ., NY
• OSTI ID: 6566478
• Journal Information: Am. J. Trop. Med. Hyg.; (United States), Journal Name: Am. J. Trop. Med. Hyg.; (United States) Vol. 29:3; ISSN AJTHA
• Country of Publication: United States
• Language: English

Related Subjects

560134* -- Radiation Effects on Microorganisms-- Vaccine Preparation & Other Applications-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CRYOGENICS
DISEASES
EVALUATION
FREEZING
IMMUNITY
INFECTIOUS DISEASES
MALARIA
MAMMALS
MICE
PARASITES
PARASITIC DISEASES
PLASMODIUM
RADIATION EFFECTS
RODENTS
SPOROZOA
VACCINES
VERTEBRATES