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Effect of 60Co-irradiation on the development and immunogenicity of Plasmodium berghei sporozoites in Anopheles stephensi mosquitoes

Journal Article · · J. Parasitol.; (United States)
OSTI ID:7004869
Protection conferred to mice by Plasmodium berghei sporozoites increased significantly when the time interval between 60Co-irradiation of the infected mosquitoes and harvest of sporozoites increased. One thousand sporozoites conferred no protection against challenge if harvested on the day of irradiation, but protected 60% of recipient mice when harvested 28 days postirradiation. When the time between feeding of mosquitoes and irradiation was varied, sporozoites from mosquitoes irradiated 3 days after feeding were infective for mice. Sporozoites from mosquitoes irradiated on day 10 postfeeding were not infective, but were immunogenic. In all experiments a decline occurred in the number of recoverable sporozoites over a 28-day period postirradiation to less than 10% of the yield on the day of irradiation.
Research Organization:
Immunoparasitology Branch, Naval Medical Research Institute, Bethesda, Maryland
OSTI ID:
7004869
Journal Information:
J. Parasitol.; (United States), Journal Name: J. Parasitol.; (United States) Vol. 69:5; ISSN JOPAA
Country of Publication:
United States
Language:
English

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