Differences in susceptibility among mouse strains to infection with Plasmodium berghei (ANKA clone) sporozoites and its relationship to protection by gamma-irradiated sporozoites

Technical Report · Mon Jan 01 04:00:00 EST 1990

OSTI ID:6483490

Jaffe, R I; Lowell, G H; Gordon, D M

Three inbred mouse strains, C57BL/6 (H-2/b/), A/J (H-2/a/), and BALB/C (H-2/d/), and one outbred strain, CD-1, demonstrated differences in susceptibility to challenge intravenously with the ANKA clone of Plasmodium berghei. Mice were challenged with 100, 1000, or 10000 sporozoites, then evaluated daily beginning on day 4 for patency. CD-1 mice were further evaluated at challenge doses of 125000, 25000, and 50000 sporozoites. C57BL/6 mice were the easiest to infect with 90% becoming infected with 100 sporozoites. The outbred strain CD-1 was the most difficult to infect requiring a challenge dose of 25000 sporozoites per mouse in order to achieve a 100% infection rate. Mouse strains also demonstrated differences in their ability to be protected by intravenous immunization with gamma-irradiated sporozoites. A/J mice needed a minimum of 3 doses of irradiated sporozoites for protection against a challenge with 1000 sporozoites. In contrast BALB/C mice, immunized with a single dose of 1000 irradiated sporozoites, are protected against a 10000 sporozoite challenge. These data suggest that both infectivity and protection are genetically restricted and that susceptibility to infection may be inversely related to protection.

• Research Organization: Walter Reed Army Inst. of Research, Washington, DC (USA). Div. of Communicable Disease and Immunology
• OSTI ID: 6483490
• Report Number(s): AD-A-224288/1/XAB
• Country of Publication: United States
• Language: English

Related Subjects

553003* -- Agriculture & Food Technology-- Pest & Disease Control-- (1987-)
60 APPLIED LIFE SCIENCES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL VARIABILITY
DISEASES
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GENETIC VARIABILITY
INFECTIOUS DISEASES
INFECTIVITY
INVERTEBRATES
IONIZING RADIATIONS
MAMMALS
MICE
MICROORGANISMS
PARASITES
PARASITIC DISEASES
PLASMODIUM
PROTOZOA
RADIATION EFFECTS
RADIATIONS
RODENTS
SPOROZOA
VACCINES
VERTEBRATES