Effect of influenza infection on the phagocytic and bactericidal activities of pulmonary macrophages
The effect of mouse-adapted influenza A/PR/8/34 virus on pulmonary macrophage function was evaluated by using an in vitro system which allowed direct virus interaction with macrophages and then separate analysis of the steps required for bacterial clearance by macrophages. Infection of macrophages with this virus resulted in the appearance of a hemagglutinating activity on the macrophage surface; expression of this activity was inhibited by amantadine, 2-deoxyglucose, and cycloheximide and by pretreatment of the virus inoculum with with ultraviolet light and specific antiserum. After influenza infection, net ingestion of viable Staphylococcus aureus by macrophage monolayers was unaltered and there was no change in the fraction of the monolayer which ingested cocci over a wide range of bacterial inputs. Influenza-infected microphages also inactivated intracellular S. aureus at a rate indistinguishable from controls. Therefore, these in vitro studies do not support the hypothesis that the defect in pulmonary antibacterial mechanisms associated with influenza infections results from a direct effect of virus infection on either the phagocytic or bactericidal activity of resistant pulmonary macarophages.
- Research Organization:
- Univ. of Iowa Hospitals, Iowa City
- OSTI ID:
- 6566464
- Journal Information:
- Infect. Immun.; (United States), Journal Name: Infect. Immun.; (United States) Vol. 26:2; ISSN INFIB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560134* -- Radiation Effects on Microorganisms-- Vaccine Preparation & Other Applications-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOHYDRATES
CELL KILLING
CONNECTIVE TISSUE CELLS
CYCLOHEXIMIDE
DRUGS
ELECTROMAGNETIC RADIATION
FUNGICIDES
GLUCOSE
HEXOSES
IN VITRO
INACTIVATION
INFLUENZA VIRUSES
MACROPHAGES
MAMMALS
MICE
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PARASITES
PESTICIDES
PHAGOCYTES
PHAGOCYTOSIS
RADIATION EFFECTS
RADIATIONS
RODENTS
SACCHARIDES
SIDE EFFECTS
SOMATIC CELLS
STAPHYLOCOCCUS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRUSES