Effect of ozone on concentrations of lysozyme in phagocytizing alveolar macrophages
Demonstration of lysozyme by the immunoperoxidase method was used to determine whether ozone-induced defects in phagocytic killing of inhaled Staphylococcus aureus by rat alveolar macrophages were associated with absence of this important bactericidal enzyme. Rats were infected with aerosols of S. aureus and then exposed for 5 hr to 2.5 ppM of ozone. Left lungs were cultured for staphylococci; right lungs were stained for lysozyme and bacteria. Compared with control animals, rats exposed to ozone showed diminished rates of bacterial killing; a larger percentage of extracellular staphylococcal clumps; and absence of lysozyme in macrophages permitting staphylococcal proliferation. These results, in which absence of enzyme activity occurred in macrophages subjected to the dual insults of ozone exposure and ingested bacteria, provide an explanation for the inability of phagocytes to kill ingested bacteria after exposure to ozone.
- Research Organization:
- Univ. of California, Davis
- OSTI ID:
- 6010667
- Journal Information:
- J. Infect. Dis.; (United States), Journal Name: J. Infect. Dis.; (United States) Vol. 143:2; ISSN JIDIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROSOLS
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CELL KILLING
COLLOIDS
CONNECTIVE TISSUE CELLS
DISPERSIONS
ENZYME ACTIVITY
ENZYMES
GLYCOSYL HYDROLASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
INHALATION
INTAKE
LUNGS
LYSOZYME
MACROPHAGES
MAMMALS
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
OZONE
PEROXIDASES
PHAGOCYTES
PORPHYRINS
RATS
RESPIRATORY SYSTEM
RODENTS
SOLS
SOMATIC CELLS
STAPHYLOCOCCUS
VERTEBRATES