Suppression and recovery of the alveolar macrophage phagocytic system during continuous exposure to 0. 5 ppm ozone

Journal Article · Wed May 01 04:00:00 EDT 1991 · Experimental Lung Research; (United States)

DOI:https://doi.org/10.3109/01902149109062864· OSTI ID:5186070

Gilmour, M I; Hmieleski, R R; Stafford, E A; Jakab, G J ^[1]

---

1. Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD (USA)

Short-term exposures to ozone (O3) are known to impair pulmonary antibacterial defenses and alveolar macrophage (AM) phagocytosis in a dose-related manner. To determine the effect of prolonged O3 exposure, Swiss mice were exposed continuously to 0.5 ppm O3. At 1, 3, 7, and 14 days, intrapulmonary killing was assessed by inhalation challenge with Staphylococcus aureus or Proteus mirabilis and by comparing the number of viable bacteria remaining in the lungs at 4 h between O3-exposed and control animals. To evaluate the effects of O3 on the functional capacity of the AMs, Fc-receptor mediated phagocytosis was assessed. Ozone exposure impaired the intrapulmonary killing of S. aureus at 1 and 3 days; however, with prolonged exposure, the bactericidal capacity of the lungs returned to normal. This trend of an initial suppression followed by recovery was reflected in the phagocytic capacity of the AMs. In contrast to S. aureus, when P. mirabilis was used as the challenge organism, O3 exposure had no suppressive effect on pulmonary bactericidal activity, which correlated with an increase in the phagocytic cell population in the lungs. Morphologic examination of the lavaged macrophages showed that after 1 day of O3 exposure, the AMs were more foamy, and contained significantly more vacuoles. There was also a significant increase in binucleated cells at 3 days. These studies demonstrate that continuous exposure to O3 modulates AM-dependent lung defenses and points to the importance of the challenge organism and exposure protocol in establishing the adverse effect of O3.

• OSTI ID: 5186070
• Journal Information: Experimental Lung Research; (United States), Journal Name: Experimental Lung Research; (United States) Vol. 17:3; ISSN 0190-2148; ISSN EXLRD
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BACTERIA
BACTERIAL DISEASES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
DOSE-RESPONSE RELATIONSHIPS
INFECTIOUS DISEASES
INHALATION
INTAKE
LAVAGE
LEUKOCYTES
LUNGS
MACROPHAGES
MAMMALS
MATERIALS
MICE
MICROORGANISMS
NEUTROPHILS
ORGANS
OZONE
PHAGOCYTES
PHAGOCYTOSIS
PROTEUS
RECOVERY
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
STAPHYLOCOCCUS
VERTEBRATES
VIABILITY