Effect of ozone on concentrations of lysozyme in phagocytizing alveolar macrophages

Journal Article · Sun Feb 01 04:00:00 EST 1981 · J. Infect. Dis.; (United States)

DOI:https://doi.org/10.1093/infdis/143.2.247· OSTI ID:5657080

Kimura, A; Goldstein, E

Demonstration of lysozyme by the immunoperoxidase method was used to determine whether ozone-induced defects in phagocytic killing of inhaled Staphylococcus aureus by rat alveolar macrophages were associated with absence of this important bactericidal enzyme. Rats were infected with aerosols of S. aureus and then exposed for 5 hr to 2.5 ppm of ozone. Left lungs were cultured for staphylococci; right lungs were stained for lysozyme and bacteria. Compared with control animals, rats exposed to ozone showed diminished rates of bacterial killing; a larger percentage of extracellular, uningested bacteria; an increased number of intracellular staphylococcal clumps; and absence of lysozyme in macrophages permitting staphylococcal proliferation. These results, in which absence of enzyme activity occurred in macrophages subjected to the dual insults of ozone exposure and ingested bacteria, provide an explanation for the inability of phagocytes to kill ingested bacteria after exposure to ozone.

• OSTI ID: 5657080
• Journal Information: J. Infect. Dis.; (United States), Journal Name: J. Infect. Dis.; (United States) Vol. 143:2; ISSN JIDIA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL EFFECTS
BODY
CONNECTIVE TISSUE CELLS
ENZYME ACTIVITY
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
LUNGS
LYSOZYME
MACROPHAGES
MAMMALS
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANS
OZONE
PHAGOCYTES
PHAGOCYTOSIS
RATS
RESPIRATORY SYSTEM
RODENTS
SENSITIVITY
SOMATIC CELLS
STAPHYLOCOCCUS
VERTEBRATES