Use of a fluorescent radiolabeled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase
A fluorescent radiolabeled triacylglycerol has been synthesized by using a fluorescent fatty acid (pyrene decanoic acid) and a radiolabeled oleic acid. This analog of the natural substrate, 1(3)pyrene decanoic-2,3 (1,2)-dioleoyl-sn-glycerol, has been tested as substrate for determining lipoprotein lipase and hepatic triacylglycerol lipase activities in post-heparin plasma. Optimal conditions for the determination of the two post-heparin plasma lipases were similar to those using radiolabeled triolein. Using this substrate, both post-heparin lipases exhibited their characteristic properties (pH optimum and effect of inhibitors) and attacked external ester bonds (1 or 3) containing pyrene decanoic and oleic acids at a similar rate.
- Research Organization:
- INSERM Unite 101, Hopital Purpan, Toulouse (France)
- OSTI ID:
- 6544794
- Journal Information:
- Lipids; (United States), Journal Name: Lipids; (United States) Vol. 23:6; ISSN LPDSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BODY
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CHEMICAL PREPARATION
CHEMICAL REACTIONS
DECANOIC ACID
DECOMPOSITION
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
FLUORESCENCE
GLANDS
HYDROLASES
HYDROLYSIS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPASE
LIPIDS
LIVER
LUMINESCENCE
LYSIS
MAMMALS
MONOCARBOXYLIC ACIDS
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RABBITS
SOLVOLYSIS
SUBSTRATES
SYNTHESIS
TRACER TECHNIQUES
TRIGLYCERIDES
TRITIUM COMPOUNDS
VERTEBRATES