Structure of the human hepatic triglyceride lipase gene

Journal Article · Tue Nov 14 04:00:00 EST 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00449a002· OSTI ID:6855577

Cai, Shengjian; Wong, D M; Chen, Sanhwan; Chan, L ^[1]

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1. Baylor College of Medicine, Houston, TX (USA)

The structure of the human hepatic triglyceride lipase gene was determined from multiple cosmid clones. All the exons, exon-intron junctions, and 845 bp of the 5{prime} and 254 bp of the 3{prime} flanking DNA were sequenced. Comparison of the exon sequences to three previously published cDNA sequences revealed differences in the sequence of the codons for residue 133, 193, 202, and 234 that may represent sequence polymorphisms. By primer extension, hepatic lipase mRNA initiates at an adenine 77 bases upstream of the translation initiation site. The hepatic lipase gene spans over 60 kb containing 9 exons and 8 introns, the latter being all located within the region encoding the mature protein. The exons are all of average size (118-234 bp). Exon 1 encodes the signal peptide, exon 4, a region that binds to the lipoprotein substrate, and exon 5, an evolutionarily highly conserved region of potential catalytic function, and exons 6 and 9 encode sequences rich in basic amino acids thought to be important in anchoring the enzyme to the endothelial surface by interacting with acidic domains of the surface glycosaminoglycans. The human lipoprotein lipase gene has been recently reported to have an identical exon-intron organization containing the analogous structural domains. The observations strongly support the common evolutionary origin of these two lipolytic enzymes.

• OSTI ID: 6855577
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 28:23; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ATP
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EVOLUTION
BODY
CARBOHYDRATES
CARBOXYLESTERASES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA SEQUENCING
ELECTROPHORESIS
ENZYMES
ESTERASES
ESTERS
GLANDS
HYDROLASES
ISOTOPES
LIGHT NUCLEI
LIPASES
LIPIDS
LIVER
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYSACCHARIDES
RADIOISOTOPES
RECOMBINANT DNA
RNA
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
TRIGLYCERIDES