Organization of the human lipoprotein lipase gene and evolution of the lipase gene family

Journal Article · Fri Dec 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.24.9647· OSTI ID:6970617

Kirchgessner, T G; Heinzmann, C; Svenson, K; Ameis, D; Lusis, A J ^[1]; Chuat, J C; Etienne, J; Guilhot, S; Pilon, C; D'Auriol, L; Galibert, F ^[2]; Schotz, M C ^[3]

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1. Univ. of California, Los Angeles (USA)
2. Laboratoire d'Hematologie Experimentale, Paris (France)
3. Univ. of California, Los Angeles (USA) Wadsworth Medical Center, Los Angeles, CA (USA)

The human lipoprotein lipase gene was cloned and characterized. It is composed of 10 exons spanning {approx} 30 kilobase. The first exon encodes the 5{prime}-untranslated region, the signal peptide plus the first two amino acids of the mature protein. The next eight exons encode the remaining 446 amino acids, and the tenth exon encodes the long 3{prime}-untranslated region of 1948 nucleotides. The lipoprotein lipase transcription start site and the sequence of the 5{prime}-flanking region were also determined. The authors compared the organization of genes for lipoprotein lipase, hepatic lipase, pancreatic lipase, and Drosophila yolk protein 1, which are members of a family of related genes. A model for the evolution of the lipase gene family is presented that involves multiple rounds of gene duplication plus exon-shuffling and intron-loss events.

• OSTI ID: 6970617
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:24; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
ARTHROPODS
BIOLOGICAL EVOLUTION
BODY
CARBOXYLESTERASES
DIGESTIVE SYSTEM
DIPTERA
DOGS
DROSOPHILA
ENDOCRINE GLANDS
ENZYMES
ESTERASES
FLIES
FRUIT FLIES
GENES
GLANDS
HYDROLASES
INSECTS
INVERTEBRATES
LIPASES
LIPIDS
LIPOPROTEINS
LIVER
MAMMALS
MAN
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PRIMATES
PROTEINS
RNA
TRANSCRIPTION
VERTEBRATES