Induction of meiotic maturation in Xenopus oocytes by 12-O-tetradecanoylphorbol 13-acetate

Journal Article · Tue Mar 31 23:00:00 EST 1987 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(87)90211-4· OSTI ID:6516407

Stith, B J; Maller, J L

Fully grown Xenopus oocytes are physiologically arrested at the G2/prophase border of the first meiotic division. Addition in vitro of progesterone or insulin causes release of the G2/prophase block and stimulates meiotic cell division of the oocyte, leading to maturation of the oocyte into an unfertilized egg. The possibility that the products of polyphosphoinositide breakdown, diacylglycerol and inositol-1,4,5-trisphosphate are involved in occyte maturation was investigated. Microinjection of IP/sub 3/ into oocytes just prior to addition of progesterone or insulin accelerated the rate of germinal vesicle breakdown (GVBD) by up to 25%. Half-maximal acceleration occurred at an intracellular IP/sub 3/ concentration of 1 ..mu..M. Treatment of oocytes with the diacylglycerol analog and tumor promoter, 12-O-tetradecanoylphorbol 13-acetate (TPA) induced GVBD in the absence of hormone. Half-maximal induction of GVBD occurred with 150 nM TPA and was blocked by pretreatment of oocytes with 10 nM cholera toxin. Microinjection of highly purified protein kinase C from rat brain oocytes did not induce maturation but markedly accelerated the rate of insulin-induced oocyte maturation. However, injection of the enzyme had no effect on progesterone action. These results indicate that protein kinase C is capable of regulating oocyte maturation of Xenopus.

• Research Organization: Univ. of Colorado School of Mines, Golden
• OSTI ID: 6516407
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 169:2; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
CARCINOGENS
CELL CYCLE
CELL DIVISION
ESTERS
GERM CELLS
HORMONES
INSULIN
KETONES
KINETICS
MEIOSIS
OOCYTES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHORBOL ESTERS
PREGNANES
PROGESTERONE
PROMOTERS
STEROID HORMONES
STEROIDS
TUMOR PROMOTERS
VERTEBRATES