The incorporation of myo-inositol into phosphatidylinositol derivatives is stimulated during hormone-induced meiotic maturation of amphibian oocytes
Journal Article
·
· Experimental Cell Research; (USA)
- Universidad de Chile, Casilla, Santiago (Chile)
The incorporation of myo-({sup 3}H)inositol into phosphatidylinositol and its phosphorylated derivatives was studied by microinjection of the radioactive precursor into Xenopus laevis oocytes. Induction of meiotic maturation of the oocytes by treatment with either progesterone one or insulin resulted in a significant increase in the incorporation of myo-({sup 3}H)inositol into the phospholipid fraction. This increase occurred 3-6 h after hormonal treatment, a time coincident with the start of the breakdown of the nuclear envelope, and requires protein synthesis. The effect of progesterone and insulin contrasts with the effect of acetylcholine, which acts through a muscarinic receptor causing the activation of phospholipase C, since the latter effector causes an increase in myo-({sup 3}H)inositol incorporation, which is more rapid and does not require protein synthesis. These results suggest that the meiotic maturation process is connected with changes in inositol metabolism in the amphibian oocyte.
- OSTI ID:
- 6250604
- Journal Information:
- Experimental Cell Research; (USA), Journal Name: Experimental Cell Research; (USA) Vol. 191:2; ISSN ECREA; ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
AMINES
AMMONIUM COMPOUNDS
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOSYNTHESIS
CARBOHYDRATES
CARBOXYLESTERASES
CELL DIVISION
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
FROGS
GERM CELLS
HORMONES
HYDROGEN COMPOUNDS
HYDROLASES
INOSITOL
INOSITOLS
INSULIN
ISOTOPE APPLICATIONS
KETONES
LIPASES
LIPIDS
METABOLISM
MITOSIS
MONOSACCHARIDES
NEUROREGULATORS
OOCYTES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PARASYMPATHOMIMETICS
PEPTIDE HORMONES
PHOSPHOLIPIDS
PREGNANES
PROGESTERONE
QUATERNARY COMPOUNDS
SACCHARIDES
STEROID HORMONES
STEROIDS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
AMINES
AMMONIUM COMPOUNDS
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOSYNTHESIS
CARBOHYDRATES
CARBOXYLESTERASES
CELL DIVISION
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
FROGS
GERM CELLS
HORMONES
HYDROGEN COMPOUNDS
HYDROLASES
INOSITOL
INOSITOLS
INSULIN
ISOTOPE APPLICATIONS
KETONES
LIPASES
LIPIDS
METABOLISM
MITOSIS
MONOSACCHARIDES
NEUROREGULATORS
OOCYTES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PARASYMPATHOMIMETICS
PEPTIDE HORMONES
PHOSPHOLIPIDS
PREGNANES
PROGESTERONE
QUATERNARY COMPOUNDS
SACCHARIDES
STEROID HORMONES
STEROIDS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES