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Coupling of inositol phospholipid hydrolysis to peptide hormone receptors expressed from adrenal and pituitary mRNA in Xenopus laevis oocytes

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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The expression of several neurotransmitter and drug receptors from injected exogenous mRNA in Xenopus laevis oocytes has been demonstrated by electrophysiological measurements of ion channel activation. The expression of specific receptors for peptide hormones in such a translation system would facilitate studies on the structure and regulation of cell-surface receptors as well as their coupling to membrane transduction mechanisms. The expression of receptors for calcium-mobilizing hormones in Xenopus oocytes was sought by analysis of phospholipid turnover in hormone-stimulated oocytes. For this purpose, Xenopus oocytes were injected with mRNA extracted from bovine adrenal and pituitary glands and incubated with myo-(/sup 3/H)inositol to label plasma-membrane phosphatidylinositol phosphates. The expression of functionally active receptors for angiotensin II (AII) and thyrotropin-releasing hormone (TRH) was demonstrated by the stimulation of (/sup 3/H)inositol phosphate production by AII and TRH in the mRNA-injected, (/sup 3/H)inositol-prelabeled oocytes. The ability of AII and TRH to act by way of newly synthesized receptors from mammalian endocrine tissues to stimulate phosphatidylinositol polyphosphate hydrolysis in Xenopus oocytes suggests a generalized and conserved mechanism of receptor coupling to the transduction mechanism responsible for activation of phospholipase C in the plasma membrane.

Research Organization:
National Institute of Child Health and Human Development, Bethesda, MD (USA)
OSTI ID:
6351265
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:24; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
AMPHIBIANS
ANGIOTENSIN
ANIMALS
AQUATIC ORGANISMS
BODY
CARBOHYDRATES
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
DRUGS
ENDOCRINE GLANDS
ESTERS
GERM CELLS
GLANDS
GLOBULINS
HORMONES
HYDROLYSIS
INOSITOL
INOSITOLS
ION EXCHANGE CHROMATOGRAPHY
LABELLED COMPOUNDS
LIPIDS
LYSIS
MEMBRANE PROTEINS
MEMBRANES
MESSENGER-RNA
MOLECULAR STRUCTURE
MONOSACCHARIDES
NUCLEIC ACIDS
OOCYTES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHOLIPIDS
PITUITARY GLAND
PROTEINS
RECEPTORS
RNA
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
TRH
TRITIUM COMPOUNDS
VASOCONSTRICTORS
VERTEBRATES