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Steroid inhibition of protein incorporation by isolated amphibian oocytes

Journal Article · · Journal of Cell Biology
DOI:https://doi.org/10.1083/jcb.61.1.26· OSTI ID:1629486
 [1];  [2];  [2]
  1. Johns Hopkins Univ., Baltimore, MD (United States). School of Hygiene and Public Health; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology Division; DOE/OSTI
  2. Johns Hopkins Univ., Baltimore, MD (United States). School of Hygiene and Public Health; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology Division
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The relationship between blood protein (vitellogenin) incorporation and nuclear maturation was studied in individual amphibian oocytes after in vitro exposure to desoxycorticosterone acetate (DOCA). Isolated Rana pipiens oocytes were incubated in vitro with radioactively labeled oocyte yolk precursor ([3H]vitellogenin) obtained from estrogenized Xenopus laevis. Incorporation of labeled vitellogenin into the oocytes continued over a 24-h period. Oocytes simultaneously exposed to DOCA and to labeled vitellogenin exhibited both inhibition of vitellogenin incorporation and stimulation of nuclear maturation and cortical changes. Inhibition of vitellogenin incorporation was observed after approximately 9 h of incubation and was correlated with the time of nuclear breakdown. Preincubation of oocytes in steroid for 9 h essentially terminated vitellogenin incorporation. Incorporation of vitellogenin occurred after removal of follicle cells from the oocyte by a short treatment with EDTA. These results demonstrate the macromolecular vitellogenin transport system remains operative in oocytes which can undergo nuclear maturation and that the steroid DOCA can affect its function. Evidence suggests that the mechanism of steroid inhibition is in part the result of inhibition of the micropinocytotic process in the oocyte cortex.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1629486
Journal Information:
Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 1 Vol. 61; ISSN 0021-9525
Publisher:
Rockefeller University PressCopyright Statement
Country of Publication:
United States
Language:
English

Cited By (2)

Risks of hormonally active pharmaceuticals to amphibians: a growing concern regarding progestagens journal November 2014
The Use of Xenopus Oocytes for the Study of Ion Channel journal January 1987

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES