Intracellular transport of vitellogenin in Xenopus oocytes: an autoradiographic study

Journal Article · Fri Dec 31 23:00:00 EST 1976 · Dev. Biol.; (United States)

DOI:https://doi.org/10.1016/0012-1606(77)90145-2· OSTI ID:7089063

Brummett, A R; Dumont, J N

The role of primordial yolk platelets (PYPs) in the transport of the yolk precursor vitellogenin to the yolk platelets in Xenopus laevis oocytes has been demonstrated by electron microscopic autoradiography. Within 20 min after exposure of the oocyte to /sup 3/H-labeled-vitello-genin, silver grains are associated with small PYPs which are formed by the fusion of endosomes. At 40 min after incorporation of /sup 3/H-labeled vitellogenin, autoradiographic silver grains are associated with larger PYPs and with the superficial layer of yolk platelets. Thus, the results demonstrate that PYPs are an intermediate in the transport of vitellogenin from endosomes to yolk platelets. These observations are consonant with the general hypothesis that vitellogenin first associates (binds) with the plasma membrane, then is incorporated by endocytosis into endosomes which fuse to form PYPs, and finally the contents of the PYPs are eventually deposited into yolk platelets.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 7089063
• Journal Information: Dev. Biol.; (United States), Journal Name: Dev. Biol.; (United States) Vol. 60; ISSN DEBIA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CELL MEMBRANES
DIFFUSION
EGGS
ELECTRON MICROSCOPY
FROGS
GERM CELLS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MEMBRANES
MICROSCOPY
NUCLEI
ODD-EVEN NUCLEI
OOCYTES
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
TRACER TECHNIQUES
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES