Origin of yolk-DNA in Xenopus laevis
Xenopus laevis serum and plasma was found to contain an average of 25 ..mu..g DNA/ml. Isolated X. laevis oocytes incubated in medium containing 25 ..mu..g DNA/ml labeled with either /sup 125/I, /sup 32/P, or /sup 14/C and from three different sources (bovine, E. coli and X. laevis), incorporated the label at an average rate of 0.11 ng mm/sup -2/ h/sup -1/. Sucrose gradient fractionation of oocytes revealed that 40 to 75% of the acid-precipitable label incorporated was associated with the yolk platelets. Additional incubations of oocytes in unlabeled medium demonstrated that the DNA incorporated into the yolk platelets was undergoing turnover; only 20% of the yolk-associated DNA was still present after a one-week incubation. Our data suggest that yolk-DNA arises by the adventitious uptake of DNA present in the maternal serum by vitellogenic oocytes.
- Research Organization:
- Univ. of Tennessee, Oak Ridge
- OSTI ID:
- 7099626
- Journal Information:
- J. Exp. Zool.; (United States), Journal Name: J. Exp. Zool.; (United States) Vol. 209:3; ISSN JEZOA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBOHYDRATES
CARBON 14
CARBON ISOTOPES
CELL CULTURES
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DISACCHARIDES
DNA
EGGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-EVEN NUCLEI
FRACTIONATION
GERM CELLS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MATERIALS
MATHEMATICS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOSYNTHESIS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
OLIGOSACCHARIDES
OOCYTES
ORGANIC COMPOUNDS
ORIGIN
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SACCHARIDES
SACCHAROSE
SEPARATION PROCESSES
STATISTICS
TIME DEPENDENCE
VERTEBRATES
YEARS LIVING RADIOISOTOPES