Development of a culture medium for growing Xeopus laevis oocytes
Journal Article
·
· Gamete Res.; (United States)
A defined medium for growing defolliculated Xenopus laevis oocytes was developed by monitoring vitellogenin incorporation. Optimum conditions were acheived by use of 50% Liebovitz L-15 medium supplemented with 1 mM L-glutamine, 15 mM Hepes, 5 mg/ml vitellogenin, and 1 ..mu..g/ml insulin (final pH = 7.8). Stage IV/V oocytes remained morphologically normal in this medium for at least two weeks and grew at an average rate of 0.25 mm/sup 3/ per week. This is the first time defolliculated Xenopus laevis oocytes have been grown in vitro.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 5028358
- Journal Information:
- Gamete Res.; (United States), Journal Name: Gamete Res.; (United States) Vol. 1; ISSN GAMRD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550300* -- Cytology
551000 -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
CULTURE MEDIA
ESTERS
GERM CELLS
GROWTH
HORMONES
HYDROGEN ISOTOPES
IN VITRO
INSULIN
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LECITHINS
LIGHT NUCLEI
LIPIDS
MORPHOLOGY
NUCLEI
NUTRIENTS
ODD-EVEN NUCLEI
OOCYTES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDE HORMONES
PHOSPHOLIPIDS
RADIOISOTOPES
REACTION KINETICS
RESPONSE MODIFYING FACTORS
TRITIUM
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES
551000 -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
CULTURE MEDIA
ESTERS
GERM CELLS
GROWTH
HORMONES
HYDROGEN ISOTOPES
IN VITRO
INSULIN
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LECITHINS
LIGHT NUCLEI
LIPIDS
MORPHOLOGY
NUCLEI
NUTRIENTS
ODD-EVEN NUCLEI
OOCYTES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PEPTIDE HORMONES
PHOSPHOLIPIDS
RADIOISOTOPES
REACTION KINETICS
RESPONSE MODIFYING FACTORS
TRITIUM
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES