Stimulation of vitellogenin uptake in Stage IV Xenopus oocytes by treatment with chorionic gonadotropin in vitro
Journal Article
·
· Biol. Reprod.; (United States)
Ovarian fragments from Xenopus laevis were incubated with various concentrations of human chorionic gonadotropin (hCG) and Stage IV oocytes were subsequently tested for their ability to incorporate vitellogenin. Such oocytes displayed incorporation rates up to 350% greater than controls. This was accompanied by increased endocytotic activity. hCG-stimulated uptake is dose dependent and reaches a maximum at 100 IU/ml, at which concentration ovulation also occurs. At 100 IU/ml of hCG, there is a lag period of at least 12 h between gonadotropin treatment and increased vitellogenin incorporation. Because hCG has little effect on isolated (dissected) cultured Stage IV oocytes which have lost their follicle cells, it is postulated that intact follicle cells are required for the induction of vitellogenin uptake.
- Research Organization:
- Oak Ridge National Lab., TN
- OSTI ID:
- 6687209
- Journal Information:
- Biol. Reprod.; (United States), Journal Name: Biol. Reprod.; (United States) Vol. 18:5; ISSN BIREB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550300* -- Cytology
550301 -- Cytology-- Tracer Techniques
550500 -- Metabolism
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CYTOLOGICAL TECHNIQUES
EGGS
ELECTRON MICROSCOPY
GAMETES
GAMETOGENESIS
GERM CELLS
GONADOTROPINS
HCG
HORMONES
HYDROGEN ISOTOPES
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MICROSCOPY
NUCLEI
ODD-EVEN NUCLEI
OOGENESIS
ORGANIC COMPOUNDS
OVA
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
STIMULATION
TRACER TECHNIQUES
TRITIUM
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550301 -- Cytology-- Tracer Techniques
550500 -- Metabolism
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CYTOLOGICAL TECHNIQUES
EGGS
ELECTRON MICROSCOPY
GAMETES
GAMETOGENESIS
GERM CELLS
GONADOTROPINS
HCG
HORMONES
HYDROGEN ISOTOPES
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MICROSCOPY
NUCLEI
ODD-EVEN NUCLEI
OOGENESIS
ORGANIC COMPOUNDS
OVA
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RADIOISOTOPES
STIMULATION
TRACER TECHNIQUES
TRITIUM
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES