Effects of protein kinase C activators on germinal vesicle breakdown and polar body emission of mouse oocytes

Journal Article · Tue Dec 31 23:00:00 EST 1985 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(86)90603-8· OSTI ID:5784820

Bornslaeger, E A; Poueymirou, W T; Mattei, P; Schultz, R M

Protein phosphorylation mediated by cAMP-dependent protein kinase is instrumental in maintaining meiotic arrest of mouse oocytes. To assess whether protein phosphorylation mediated by calcium/phospholipid-dependent protein kinase (protein kinase C) might also inhibit the resumption of meiosis, oocytes were treated with activators of this enzyme. The active phorbol esters 12-O-tetra-decanoyl phorbol-13-acetate (TPA) and 4..beta..-phorbol, 12,13-didecanoate (4..beta..-PDD) inhibited germinal vesicle breakdown (GVBD), as did a more natural activator of protein kinase, C, sn-1,2-dioctanoylglycerol (diC/sub 8/). An inactive phorbol ester, 4a-phorbol 12,13-didecanoate (4..cap alpha..-PDD), did not inhibit GVBD. TPA did not inhibit the maturation-associated decrease in oocyte cAMP. Microinjected heat-stable protein inhibitor of a cAMP-dependent protein kinase failed to induce GVBD in the presence of TPA. Both TPA and diC/sub 8/ partially inhibited specific changes in oocyte phosphoprotein metabolism that are tightly correlated with resumption of meiosis; these agents also induced the apparent phosphorylation of specific oocyte proteins. These results suggest that protein kinase C activators may inhibit resumption of meiosis by acting distal to a decrease in cAMP-dependent protein kinase activity, but prior to changes in oocyte phosphoprotein metabolism that are presumably required for resumption of meiosis.

• Research Organization: Univ. of Pennsylvania, Philadelphia
• OSTI ID: 5784820
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 165; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550300 -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
AMP
ANIMALS
BIOLOGICAL EFFECTS
CALCIUM
CARCINOGENS
CELL CYCLE
CELL DIVISION
CHEMICAL REACTIONS
ELEMENTS
ENZYMES
ESTERS
GERM CELLS
LIPIDS
MAMMALS
MEIOSIS
METALS
MICE
NUCLEOTIDES
OOCYTES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHORBOL ESTERS
PHOSPHOLIPIDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
RODENTS
TRANSFERASES
VERTEBRATES