Characterization of a new monoclonal antibody against mercury (II)

Journal Article · Wed Jul 01 04:00:00 EDT 1998 · Analytical Letters

DOI:https://doi.org/10.1080/00032719808005248· OSTI ID:651045

Marx, A; Hock, B ^[1]

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1. Technische Univ. Muenchen, Freising (Germany). Dept. of Botany

Monoclonal antibodies (mabs) were produced against mercury (II) and an enzyme immunoassay was developed for the detection of mercury (II) in water. Since mercury (II) ions are too small to elicit an immune response, they were coupled via glutathione (GSH) to the immunogenic carrier protein keyhole limpet hemocyanin (KLH). Several mice were immunized with this KLH-GSH-Hg immunoconjugate. Spleen cells of immunized mice were fused with myeloma cells. The resulting hybridoma cells were screened for the production of specific anti-Hg mabs. Five positive cells were detected. The hybridoma cell line K3C6 was adjusted to protein free medium; it produced mabs with high selectivity and sensitivity. A detection limit of 2.8 {micro}g/L HgCl{sub 2} (= 2.1 {micro}g/L Hg{sup 2+}) was achieved with a non-competitive enzyme immunoassay (EIA). Cross-reactivities with other metals were below 1%. Measurement of spiked water samples with this EIA showed good correlation with results obtained by mass spectrometry with inductive coupled plasma (ICP-MS).

• Sponsoring Organization: USDOE
• OSTI ID: 651045
• Journal Information: Analytical Letters, Journal Name: Analytical Letters Journal Issue: 10 Vol. 31; ISSN ANALBP; ISSN 0003-2719
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
54 ENVIRONMENTAL SCIENCES
ANTIBODIES
CHEMICAL ANALYSIS
CONTAMINATION
ENVIRONMENT
ENZYMES
EXPERIMENTAL DATA
IMMUNOASSAY
MERCURY
VALIDATION