Photoregulation process of sorghum leaf phosphoenolpyruvate carboxylase: study with monoclonal antibodies

Journal Article · Fri Feb 27 04:00:00 EST 1987 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(87)90646-2· OSTI ID:6577341

Thomas, M; Cretin, C; Keryer, E; Vidal, J; Gadal, P; Bidart, J M; Buhuon, C

Monoclonal antibodies were produced against the G isozyme subunit of PEP carboxylase (PEPC) from Sorghum leaves by the hybridoma technique. More than 400 antibodies-producing hybridomas to PEPC were produced from the fusion of spleen cells from immunized mice with NS1 myeloma cells. By using an ELISA, three hybridomas (91-G, 83-G, 49-EG) were selected. Monoclonal antibodies were subsequently characterized in a Western experiment; Mabs 83-G and 91-G were found to be highly specific to the G isozyme whereas Mab 49-EG recognized both forms (E and G isozymes) of the enzyme. Addition of Mabs to the enzyme preparation did not modify its catalytic activity nor its activation by glycine. Use of these probes provided direct and definite evidence of the specific enhancing effect of light on the G form and on its corresponding mRNA.

• Research Organization: Universite de Paris-Sud, Orsay, France
• OSTI ID: 6577341
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 1; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLASE
CEREALS
CHEMICAL REACTIONS
CHEMISTRY
ENZYME IMMUNOASSAY
ENZYMES
GRASS
HYBRIDOMAS
IMMUNOASSAY
ISOENZYMES
KINETICS
LYASES
MONOCLONAL ANTIBODIES
PHOTOCHEMICAL REACTIONS
PHOTOCHEMISTRY
PHOTOSYNTHESIS
PLANTS
REACTION KINETICS
SOMATIC CELLS
SORGHUM
SPLEEN CELLS
SYNTHESIS