Murine lung immunity to a soluble antigen

Journal Article · Mon Jan 01 04:00:00 EST 1990 · American Journal of Respiratory Cell and Molecular Biology; (United States)

DOI:https://doi.org/10.1165/ajrcmb/2.4.327· OSTI ID:7160143

Weissman, D N; Bice, D E; Siegel, D W; Schuyler, M R ^[1]

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1. Albuquerque Veterans Administration Medical Center, NM (United States) Lovelace Biomedical and Environmental Research Inst., Albuquerque, NM (United States)

To test the hypothesis that soluble antigen triggers antigen-specific immunity in the respiratory tract in a fashion similar to that reported for particulate antigen, the authors examined the development of local and systemic immunity in C57BL/6 mice after intratracheal (i.t.) instillation of a soluble, large molecular weight protein neoantigen, keyhole limpet hemocyanin (KLH). Specific anti-KLH IgG and IgM first appeared in the sera of mice on day 7 after primary immunization by i.t. instillation of KLH, with specific serum antibody concentrations remaining elevated at day 11. Cultured spleen cells obtained from mice after primary immunization released only low levels of specific IgM, and no specific IgG. No specific antibody was released by cell populations derived from the lungs of animals undergoing primary immunization. When presensitized mice were given an i.t. challenge with KLH, responses differed markedly from those following primary immunization. Lung-associated lymph node cell populations from challenged mice released greater amounts of specific antibody earlier than did cell populations, which after primary immunization had not released detectable amounts of specific antibody in vitro, released easily detectable amounts of specific antibody after challenge. Thus, i.t. instillation of soluble KLH generates specific immunity in mice in a fashion similar to that reported for particulate antigen. Specific responses following primary immunization occur largely within draining lung-associated lymph nodes. In contrast, presensitized animals challenged i.t. with soluble KLH mount secondary antibody responses in both lung and lung-associated lymph nodes.

• DOE Contract Number: AC04-76EV01013
• OSTI ID: 7160143
• Journal Information: American Journal of Respiratory Cell and Molecular Biology; (United States), Journal Name: American Journal of Respiratory Cell and Molecular Biology; (United States) Vol. 2; ISSN 1044-1549; ISSN AJRBE
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
BIOLOGICAL FUNCTIONS
BODY
DATA
EXPERIMENTAL DATA
IMMUNE REACTIONS
IMMUNITY
INFORMATION
LUNGS
MAMMALS
MICE
NUMERICAL DATA
ORGANS
RESPIRATORY SYSTEM
RODENTS
SOLUBILITY
SOMATIC CELLS
SPLEEN CELLS
VERTEBRATES