Early events in herpes simplex virus type 1 infection: photosensitivity of fluorescein isothiocyanate-treated virions

Journal Article · Sat Jan 31 23:00:00 EST 1981 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.78.2.912· OSTI ID:6501521

DeLuca, N; Bzik, D; Person, S; Snipes, W

Herpes simplex virus type 1 is photosensitized by treatment with fluorescein isothiocyanate (FITC). The inactivation of FITC-treated virions upon subsequent exposure to light is inhibited by the presence of sodium azide, suggesting the involvement of singlet oxygen in the process. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis revealed that treatment with FITC plus light induces crosslinks in viral envelope glycoproteins. Treatment of virions with high concentrations of FITC (50 ..mu..g/ml) plus light causes a reduction in the adsorption of the virus to monolayers of human embryonic lung cells. For lower concentrations of FITC (10 ..mu..g/ml) plus light, treated virions adsorb to the host cells, but remain sensitive to light until entry occurs. The loss of light sensitivity coincides with the development of resistance to antibodies. These results are most consistent with a mechanism of entry for herpes simplex virus involving fusion of the viral membrane with the plasma membrane of the host cell.

• Research Organization: Pennsylvania State Univ., University Park
• OSTI ID: 6501521
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 78:2; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ADDITIVES
ADSORPTION
ANIMAL CELLS
ANTIBODIES
ANTITHYROID DRUGS
AROMATICS
AZIDES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOHYDRATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CHEMICAL REACTIONS
CROSS-LINKING
DATA
DETERGENTS
DISEASES
DRUGS
DYES
ELECTROPHORESIS
EMULSIFIERS
EXPERIMENTAL DATA
FLUORESCEIN
GLUCOPROTEINS
HERPES SIMPLEX
HORMONE ANTAGONISTS
HYDROXY ACIDS
HYDROXY COMPOUNDS
INACTIVATION
INFECTIOUS DISEASES
INFECTIVITY
INFORMATION
MEMBRANES
MICROORGANISMS
NITROGEN COMPOUNDS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PHENOLS
PHOTOREACTIVATION
PHOTOSENSITIVITY
POLYMERIZATION
POLYPHENOLS
PROTEINS
RECOVERY
REPAIR
SACCHARIDES
SENSITIVITY
SKIN DISEASES
SORPTION
SURFACTANTS
SURVIVAL CURVES
THIOCYANATES
VIRAL DISEASES
VIRUSES
WETTING AGENTS