Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells

Journal Article · Mon Jun 01 00:00:00 EDT 1981 · Infect. Immun.; (United States)

OSTI ID:5576958

Redfield, D C; Richman, D D; Oxman, M N; Kronenberg, L H

Psoralen compounds covalently bind to nucleic acids when irradiated with long-wavelength ultraviolet light. This treatment can destroy the infectivity of deoxyribonucleic acid and ribonucleic acid viruses. Two psoralen compounds, 4'-hydroxymethyltrioxsalen and 4'-aminomethyltrioxsalen, were used with long-wavelength ultraviolet light to inactivate cell-free herpes simplex and influenza viruses and to render virus-infected cells noninfectious. This method of inactivation was compared with germicidal (short-wavelength) ultraviolet light irradiation. The antigenicity of the treated, virus-infected, antigen-bearing cells was examined by immunofluorescence and radioimmunoassay and by measuring the capacity of the herpes simplex virus-infected cells to stimulate virus-specific lymphocyte proliferation. The infectivity of the virus-infected cells could be totally eliminated without altering their viral antigenicity. The use of psoralen plus long-wavelength ultraviolet light is well suited to the preparation of noninfectious virus antigens and virus antigen-bearing cells for immunological assays.

• OSTI ID: 5576958
• Journal Information: Infect. Immun.; (United States), Vol. 32:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
CHEMICAL PREPARATION
RADIOIMMUNOASSAY
HERPES SIMPLEX
INACTIVATION
RADIOINDUCTION
INFLUENZA VIRUSES
PSORALEN
BIOLOGICAL EFFECTS
CELL PROLIFERATION
FLUORESCENCE
LYMPHOCYTES
NEAR ULTRAVIOLET RADIATION
ANIMAL CELLS
ANTICOAGULANTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
COUMARINS
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LEUKOCYTES
LUMINESCENCE
MATERIALS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PARASITES
RADIATIONS
RADIOASSAY
SKIN DISEASES
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
VIRAL DISEASES
VIRUSES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
550700 - Microbiology