Human cytomegalovirus renders cells non-permissive for replication of herpes simplex viruses

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:5713971

Cockley, K D

The herpes simplex virus (HSV) genome during production infection in vitro may be subject to negative regulation which results in modification of the cascade of expression of herpes virus macromolecular synthesis leading to establishment of HSV latency. In the present study, human embryonic lung (HEL) cells infected with human cytomegalovirus (HCMV) restricted the replication of HSV type-1 (HSV-1). A delay in HSV replication of 15 hr as well as a consistent, almost 1000-fold inhibition of HSV replication in HCMV-infected cell cultures harvested 24 to 72 hr after superinfection were observed compared with controls infected with HSV alone. HSV type-2 (HSV-2) replication was similarly inhibited in HCMV-infected HEL cells. Prior ultraviolet-irradiation (UV) of HCMV removed the block to HSV replication, demonstrating the requirement for an active HCMV genome. HCMV deoxyribonucleic acid (DNA) negative temperature-sensitive (ts) mutants inhibited HSV replications as efficiently as wild-type (wt) HCMV at the non-permissive temperature. Evidence for penetration and replication of superinfecting HSV into HCMV-infected cells was provided by blot hybridization of HSV DNA synthesized in HSV-superinfected cell cultures and by cesium chloride density gradient analysis of ({sup 3}H)-labeled HSV-1-superinfected cells.

• Research Organization: Pennsylvania State Univ., University Park, PA (USA)
• OSTI ID: 5713971
• Country of Publication: United States
• Language: English

Related Subjects

550901 -- Pathology-- Tracer Techniques
560130* -- Radiation Effects on Microorganisms
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
DISEASES
DNA HYBRIDIZATION
DNA REPLICATION
ELECTROMAGNETIC RADIATION
EMBRYOS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HERPES SIMPLEX
HYBRIDIZATION
HYDROGEN COMPOUNDS
INFECTIOUS DISEASES
INFECTIVITY
ISOTOPE APPLICATIONS
LUNGS
MAMMALS
MAN
MICROORGANISMS
NUCLEIC ACID REPLICATION
ORGANS
PARASITES
PRIMATES
RADIATION EFFECTS
RADIATIONS
RESPIRATORY SYSTEM
SKIN DISEASES
TEMPERATURE DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES
VIRUSES