Enhanced capacity of DNA repair in human cytomegalovirus-infected cells

Journal Article · Wed Apr 01 04:00:00 EST 1981 · J. Virol.; (United States)

OSTI ID:5285011

Nishiyama, Y; Rapp, F

Plaque formation in Vero cells by UV-irradiated herpes simplex virus was enhanced by infection with human cytomegalovirus (HCMV), UV irradiation, or treatment with methylmethanesulfonate. Preinfection of Vero cells with HCMV enhanced reactivation of UV-irradiated herpes simplex virus more significantly than did treatment with UV or methylmethanesulfonate alone. A similar enhancement by HCMV was observed in human embryonic fibroblasts, but not in xeroderma pigmentosum (XP12BE) cells. It was also found that HCMV infection enhanced hydroxyurea-resistant DNA synthesis induced by UV light or methylmethanesulfonate. Alkaline sucrose gradient sedimentation analysis revealed an enhanced rate of synthesis of all size classes of DNA in UV-irradiated HCMV-infected Vero cells. However, HCMV infection did not induce repairable lesions in cellular DNA and did not significantly inhibit host cell DNA synthesis, unlike UV or methylmethanesulfonate. These results indicate that HCMV enhanced DNA repair capacity in the host cells without producing detectable lesions in cellular DNA and without inhibiting DNA synthesis. This repair appeared to be error proof for UV-damaged herpes simplex virus DNA when tested with herpes simplex virus thymidine kinase-negative mutants.

• OSTI ID: 5285011
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 38:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

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550400 -- Genetics
550700 -- Microbiology
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOSYNTHESIS
CELL CULTURES
COMPARATIVE EVALUATIONS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ESTERS
HERPES SIMPLEX
INFECTIOUS DISEASES
METHYL METHANESULFONATE
MUTAGENS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLAQUE FORMATION
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SKIN DISEASES
SULFONIC ACID ESTERS
SYNTHESIS
ULTRAVIOLET RADIATION
VIRAL DISEASES
XERODERMA PIGMENTOSUM