Human cytomegalovirus function inhibits replication of herpes simplex virus

Journal Article · Fri Jan 01 04:00:00 EST 1988 · J. Virol.; (United States)

OSTI ID:5431104

Cockley, K D; Shiraki, K; Rapp, F

Human embryonic lung (HEL) cells infected with human cytomegalovirus (HCMV) restricted the replication of herpes simplex virus type 1 (HSV-1). A delay in HSV replication of 15 h as well as a consistent, almost 3 log inhibition of HSV replication in HCMV-infected cell cultures harvested 24 to 72 h after superinfection were observed compared with controls infected with HSV alone. Treatment of HCMV-infected HEL cells with cycloheximide (100 ..mu..g/ml) for 3 or 24 h was demonstrated effective in blocking HCMV protein synthesis, as shown by immunoprecipitation with HCMV antibody-positive polyvalent serum. Cycloheximide treatment of HCMV-infected HEL cells and removal of the cycloheximide block before superinfection inhibited HSV-1 replication more efficiently than non-drug-treated superinfected controls. HCMV DNA-negative temperature-sensitive mutants restricted HSV as efficiently as wild-type HCMV suggesting that immediate-early and/or early events which occur before viral DNA synthesis are sufficient for inhibition of HSV. Inhibition of HSV-1 in HCMV-infected HEL cells was unaffected by elevated temperature (40.5/sup 0/C). However, prior UV irradiation of HCMV removed the block to HSV replication, demonstrating the requirement for an active HCMV genome. HSV-2 replication was similarly inhibited in HCMV-infected HEL cells. Superinfection of HCMV-infected HEL cells with HSV-1 labeled with (/sup 3/H)thymidine provided evidence that the labeled virus could penetrate to the nucleus of cells after superinfection. Evidence for penetration of superinfecting HSV into HCMV-infected cells was also provided by blot hybridization of HSV DNA synthesized in cells infected with HSV alone versus superinfected cell cultures at 0 and 48 h after superinfection.

• Research Organization: Pennsylvania State Univ. College of Medicine, Hershey
• OSTI ID: 5431104
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 62:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
EMBRYONIC CELLS
EVEN-ODD NUCLEI
HERPES SIMPLEX
HYBRIDIZATION
INFECTIOUS DISEASES
INHIBITION
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
LUNGS
MAMMALS
MAN
METHIONINE
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PARASITES
PRIMATES
PURIFICATION
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RESPIRATORY SYSTEM
SKIN DISEASES
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES
VIRUSES