Membrane-membrane interactions in a lipid-containing bacteriophage system. Progress report, October 1, 1980-September 30, 1981
Virus-cell interactions and the mechanism of viral entry have been the major focal points of this research. A method of analysis was perfected to investigate the entry process for herpes simplex virus. This technique makes use of a photosensitizing dye, FITC, that covalently binds to viral envelope proteins. Treated virions remain photosensitive until the envelope is shed during the process of infection. Our data strongly support an entry mechanism in which the viral envelope fuses with the cell plasma membrane. Other related projects have involved studies of the virucidal properties of retinoids, plaque development characteristics for viruses surviving treatment with membrane perturbers, and a large plaque effect that occurs when virus are plated on cells pretreated with uv light. In addition, we have characterized a new bacteriophage, investigated the interactions of divalent cations and proteins with phospholipid vesicles, extended our studies of the effects of hydrophobic photosensitizers on cell membranes, and used the spin-trapping technique to elucidate the reaction mechanism for an enzyme-like activity in soil extracts.
- Research Organization:
- Pennsylvania State Univ., University Park (USA). Dept. of Microbiology and Cell Biology
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AS02-76EV02311
- OSTI ID:
- 6503241
- Report Number(s):
- DOE/EV/02311-32
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BACTERIOPHAGES
CELL CONSTITUENTS
CELL MEMBRANES
DISEASES
ESTERS
HERPES SIMPLEX
INFECTIOUS DISEASES
INFECTIVITY
INTERACTIONS
LIPIDS
MEMBRANES
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PARASITES
PHOSPHOLIPIDS
PLAQUE FORMATION
RESEARCH PROGRAMS
SKIN DISEASES
VIRAL DISEASES
VIRUSES