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Coordinate induction of metallothioneins I and II in rodent cells by UV irradiation

Journal Article · · Mol. Cell. Biol.; (United States)
; ;
Sequence analysis of Chinese hamster V79 lung fibroblast cDNA clones, which code for UV radiation-inducible transcripts, revealed that many of the clones corresponded to metallothioneins (MTs) I and II. A third cDNA clone, DDIU4, was found also to code for a similar-size UV-inducible transcript which was unrelated to MT by both sequence analysis and kinetics of induction. MTI and MTII RNAs rapidly increased in V79 cells within 1 h after UV irradiation, and maximum induction was seen by 4 h. This rapid induction of MT RNA by UV irradiation was not observed in human fibroblasts. MTI and MTII were coordinately induced in both time course and dose-response experiments, although the induction of MTII, up to 30-fold, was three to four times greater than that of MTI. The induction of MT did not appear to be a general stress response, since no increase occurred after exposure to X rays or H/sub 2/O/sub 2/.
Research Organization:
National Cancer Institute, Bethesda, MD (USA)
OSTI ID:
6496718
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:11; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
CLONING
CONNECTIVE TISSUE CELLS
DNA
DNA HYBRIDIZATION
DNA-CLONING
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HAMSTERS
HYBRIDIZATION
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
IONIZING RADIATIONS
LUNGS
MAMMALS
METALLOPROTEINS
METALLOTHIONEIN
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PEROXIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RESPIRATORY SYSTEM
RNA
RODENTS
SOMATIC CELLS
SYNTHESIS
TRANSCRIPTION
ULTRAVIOLET RADIATION
VERTEBRATES
X RADIATION