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DNA damage-inducible transcripts in mammalian cells

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ;
Hybridization subtraction at low ratios of RNA to cDNA was used to enrich for the cDNA of transcripts increased in Chinese hamster cells after UV irradiation. Forty-nine different cDNA clones were isolated. Most coded for nonabundant transcripts rapidly induced 2- to 10-fold after UV irradiation. Only 2 of the 20 cDNA clones sequenced matched known sequences (metallothionein I and II). The predicted amino acid sequence of one cDNA had two localized areas of homology with the rat helix-destabilizing protein. These areas of homology were at the two DNA-binding sites of this nucleic acid single-strand-binding protein. The induced transcripts were separated into two general classes. Class I transcripts were induced by UV radiation and not by the alkylating agent methyl methanesulfonate. Class II transcripts were induced by UV radiation and by methyl methanesulfonate. Many class II transcripts were induced also by H2O2 and various alkylating agents but not by heat shock, phorbol 12-tetradecanoate 13-acetate, or DNA-damaging agents which do not produce high levels of base damage. Since many of the cDNA clones coded for transcripts which were induced rapidly and only by certain types of DNA-damaging agents, their induction is likely a specific response to such damage rather than a general response to cell injury.
Research Organization:
National Cancer Institute, Bethesda, MD (USA)
OSTI ID:
6565958
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:23; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMINO ACID SEQUENCE
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARCINOGENS
CHO CELLS
CLONING
DNA
DNA HYBRIDIZATION
DNA-CLONING
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ESTERS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HYBRIDIZATION
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
METHYL METHANESULFONATE
MOLECULAR STRUCTURE
MUTAGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
PHORBOL ESTERS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
SULFONIC ACID ESTERS
TRANSCRIPTION
ULTRAVIOLET RADIATION