Coordinate amplification of metallothionein I and II genes in cadmium-resistant Chinese hamster cells: implications for mechanisms regulating metallothionein gene expression

Journal Article · Fri Feb 01 04:00:00 EST 1985 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.5.2.320· OSTI ID:6178403

Crawford, B D; Enger, M D; Griffith, B B; Griffith, J K; Hanners, J L; Longmire, J L; Munk, A C; Stallings, R L; Tesmer, J G; Walters, R A; Hildebrand, C E

The authors describe here the derivation, characterization, and use of clonal cadmium-resistance (Cd/sup r) strains of the Chinese hamster cell line CHO which differ in their metallothionein (MT) induction capacity. By nondenaturing polyacrylaminde gel electrophoresis, the authors showed that the stable Cd/sup r/ phenotype is correlated with the augmented expression of both isometallothioneins (MTI and MTII). In cells resistant to concentrations of CdCl2 exceeding 20 M, coordinate amplifications of genes encoding both isometallothioneins was demonstrated by using cDNA MT-coding sequence probes and probes specific for 3'-noncoding regions of Chinese hamster MTI and MTII genes. Molecular and in situ hybridization analyses supported close linkage of Chinese hamster MTI and MTII genes, which the authors have mapped previously to Chinese hamster chromosome 3. This suggests the existence of a functionally related MT gene cluster in this species. Amplified Cd/sup r/ variants expressing abundant MT and their corresponding Cd/sup s/ parental CHO cells should be useful for future studies directed toward elucidating the mechanisms that regulate expressions of the isometallothioneins. 59 references, 8 figures.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 6178403
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 5:2; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
CADMIUM
CHO CELLS
CHROMOSOMES
DATA
DNA SEQUENCING
ELECTROPHORESIS
ELEMENTS
EXPERIMENTAL DATA
GENE REGULATION
GENES
HYBRIDIZATION
INFORMATION
METALLOPROTEINS
METALLOTHIONEIN
METALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PHENOTYPE
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TOLERANCE