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Cadmium-resistant variant of the Chinese hamster (CHO) cell with increased metallothionein induction capacity

Journal Article · · Exp. Cell Res.; (United States)
The toxic trace metal Cd/sup 2 +/ has been used to select a variant (designated Cd/sup r/) of the Chinese hamster cell (line CHO) resistant to the growth-inhibitory and cytotoxic effects of Cd/sup 2 +/. Comparison of the relative capacities of these two cell types to induce specific low molecular weight Cd/sup 2 +/-binding proteins (metallothioneins) reveals that the Cd/sup r/ cell has an increased capacity to induce metallothionein and to sequester intracellular Cd/sup 2 +/ in metallothioneins. These results suggest that the greater competence of the Cd/sup r/ cell to induce metallothionein is a major factor in the Cd/sup 2 +/-resistant phenotype of the variant.
Research Organization:
Los Alamos Scientific Lab., NM
OSTI ID:
6863556
Journal Information:
Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 124; ISSN ECREA
Country of Publication:
United States
Language:
English