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Comparative studies of zinc metabolism in cultured Chinese Hamster cells wth differing metallothionein-induction capacities

Journal Article · · Biol. Trace Elem. Res.; (United States)
DOI:https://doi.org/10.1007/BF02783822· OSTI ID:6279335
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Previous work in our laboratory led to the isolation of a cadmium (Cd)-resistant variant (Cd/sup r/2C10) of the line CHO Chinese Hamster cell having a 10-fold greater resistance to the cytotoxic action of Cd/sup 2 +/ compared with the CHO cell. Evidence that Cd/sup 2 +/ behaves as an analog of the essential trace metal, Zn/sup 2 +/, especially as an inducer of MT synthesis, suggested that the Cd/sup r/ and CHO cell types could be employed to investigate cellular Zn/sup 2 +/ metabolism. In the present study, measurements were made to compare CHO and Cd/sup r/ cell types for (a) growth as a function of the level of ZnCl/sub 2/ added to the culture medium, (b) uptake and subcellular distribution of Zn/sup 2 +/, and (c) capacity to induce MT synthesis. These findings suggest that (a) the CHO and Cd/sup r/ cell systems will be useful in further studies of cellular Zn/sup 2 +/ metabolism, especially in comparisons of Zn/sup 2 +/ metabolism in the presence and absence of induction of the Zn/sup 2 +/-sequestering MT and (b) a relationship exists between cellular capacity to induce MT synthesis and capacity for cellular Zn/sup 2 +/ uptake.
Research Organization:
Los Alamos Scientific Lab., NM
OSTI ID:
6279335
Journal Information:
Biol. Trace Elem. Res.; (United States), Journal Name: Biol. Trace Elem. Res.; (United States) Vol. 2; ISSN BTERD
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CHEMICAL ACTIVATION
CHLORIDES
CHLORINE COMPOUNDS
CONNECTIVE TISSUE CELLS
FIBROBLASTS
HALIDES
HALOGEN COMPOUNDS
HAMSTERS
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SOMATIC CELLS
VERTEBRATES
ZINC CHLORIDES
ZINC COMPOUNDS
ZINC HALIDES