Assignment of genes encoding metallothioneins I and II to Chinese hamster chromosomes 3. Evidence for the role of chromosome rearrangement in gene amplification

Journal Article · Sat Dec 01 04:00:00 EST 1984 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.4.12.2932· OSTI ID:5804834

Stallings, R L; Munk, A C; Longmire, J L; Hildebrand, C E; Crawford, B D

Cadmium resistant (Cd/sup r/) variants with coordinately amplified metallothionein I and II (MTI and MTII) genes have been derived from both Chinese hamster ovary and near-euploid Chinese hamster cell lines. Cytogenetic analyses of Cd/sup r/ variants consistently revealed breakage and rearrangement involving chromosome 3p. In situ hybridization with Chinese hamster MT-encoding cDNA probe localized amplified MT gene sequences near the translocation breakpoint involving chromosome 3p. These observations suggested that both functionally related, isometallothionein loci are linked on Chinese hamster chromosome 3. Southern blot analyses of DNAs isolated from a panel of Chinese hamster x mouse somatic cell hybrids which segregate hamster chromosomes confirmed that both MTI and MTII are located on chromosome 3. The authors speculate that rearrangement of chromosome 3p could be causally involved with the amplification of MT genes in Cd/sup r/ hamster cell lines. 34 references, 3 figures, 1 table.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 5804834
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 4:12; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CADMIUM
CHO CELLS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CLONING
DNA SEQUENCING
DNA-CLONING
ELEMENTS
GENES
GENETIC EFFECTS
GENETIC MAPPING
HAMSTERS
HYBRIDIZATION
MAMMALS
MAPPING
METALLOPROTEINS
METALLOTHIONEIN
METALS
MICE
MUTATIONS
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SOMATIC CELLS
STRAND BREAKS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES