Transcriptional and posttranscriptional regulation of the proliferating cell nuclear antigen gene

Journal Article · Sun Jul 01 04:00:00 EDT 1990 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.10.7.3289· OSTI ID:6485833

Chang, C D; Ottavio, L; Travali, S; Lipson, K E; Baserga, R ^[1]

---

1. Temple Univ., Philadelphia, PA (USA). School of Medicine

The steady-state mRNA levels of the proliferating cell nuclear antigen (PCNA) gene are growth regulated. In a previous paper the authors reported that introns (especially intron 4) participate in growth regulation of the PCNA gene. They have now investigated the role of the 5{prime}-flanking sequence of the human PCNA gene stably transfected into BALB/c 3T3 cells. Promoters of different lengths (from{minus}2856 to {minus}45 upstream of the cap site) were tested. All promoters except the Aatll promoter ({minus}45), including a short HpaII promoter ({minus}210), were sufficient for a response to serum, platelet-derived growth factor, and to a lesser extent epidermal growth factor. No construct responded to insulin or platelet-poor plasma. The AatII promoter had little detectable activity. Transcriptional activity was also determined in BALB/c 3T3 cells carrying various constructs of the human PCNA gene by two methods: run-on transcription and reverse transcription-polymerase chain reaction (the latter measuring the heterogeneous nuclear RNA (hnRNA) steady-state levels).

• OSTI ID: 6485833
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 10:7; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550300 -- Cytology
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CELL PROLIFERATION
ENZYMES
GENE REGULATION
GENETIC ENGINEERING
GROWTH FACTORS
MATERIALS
MESSENGER-RNA
MITOGENS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
POST-TRANSLATION MODIFICATION
PROMOTERS
PROTEINS
RNA
RNA POLYMERASES
TRANSCRIPTION
TRANSFERASES
TUMOR PROMOTERS