The promoter of the human proliferating cell nuclear antigen (PCNA) gene is bidirectional

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(90)90172-7· OSTI ID:5377961

Rizzo, M G; Ottavio, L; Travali, S; Chang, Chungder; Kaminska, B; Baserga, R ^[1]

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1. Temple Univ., Philadelphia, PA (United States)

The proliferating cell nuclear antigen (PCNA) gene codes for a protein that is necessary for cellular DNA synthesis and cell cycle progression. A functional promoter has been identified in the 5{prime} flanking region of the human PCNA gene. An abbreviated promoter was found to be equally efficient in directing transcription from a linked reporter, whether placed in the correct or reverse orientation in respect to the coding sequence. The reporter used was a cDNA of human thymidine kinase (TK), and the bidirectionality of the promoter was demonstrated by its ability to confer the TK{sup +} phenotype to TK{sup {minus}}ts13 cells and by the amount of specific message in RNA blots. The PvuII promoter placed between two coding sequences is capable of driving transcription simultaneously in both directions. Finally, in blots of RNA from human cells, two transcripts could be detected that hybridized to a sense riboprobe from the 5{prime} flanking region of the human PCNA gene. The authors conclude that the locus for the human PCNA gene contains a bidirectional promoter producing diverging transcripts.

• OSTI ID: 5377961
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 188:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
DNA
DNA POLYMERASES
DNA REPLICATION
DNA SEQUENCING
ENZYMES
GENE REPRESSORS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHENOTYPE
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYMERASES
PROMOTERS
PROTEINS
RECOMBINANT DNA
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TRANSFERASES