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Functional analysis and growth factor regulation of the human vimentin promoter

Journal Article · · Mol. Cell. Biol.; (United States)
;

Vimentin is a growth-regulated gene whose mRNA levels increase severalfold after stimulation of quiescent cells. The authors isolated and sequenced a genomic fragment of human DNA containing the vimentin 5'-flanking sequence and untranslated region. S1 nuclease analysis was used to determine the transcription initiation site. Deletion mutants of the promoter region were constructed, linked to a chloramphenicol acetyltransferase gene, and analyzed for transient expression by transfection into BALB/c 3T3 cells. These experiments revealed the presence in the human vimentin promoter region of a negative-regulatory element, flanked by positive elements. The most 5' of the positive elements is able to overcome the effects of the negative element. Analysis of these deletion constructs in stable cell lines confirmed the results of the transient assays. Using these stable cell lines, they can also demonstrate that the vimentin promoter region can confer platelet-derived growth factor inducibility to a linked chloramphenicol acetyltransferase gene and that the sequences required for this inducibility reside between positions - 241 and +73.

Research Organization:
American Cyanamid Co., Agricultural Research Div., Princeton, NJ 08540 (USA)
OSTI ID:
6971118
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 7:11; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ACETYL RADICALS
ACYL RADICALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CELL CULTURES
CHLORAMPHENICOL
DATA
DRUGS
ENZYMES
EXPERIMENTAL DATA
FUNCTIONS
GENE OPERONS
GENE REGULATION
GENES
GROWTH FACTORS
INFORMATION
MATERIALS
MESSENGER-RNA
MITOGENS
MUTANTS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PHENOTYPE
PROTEINS
RADICALS
RNA
TRANSFERASES