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Negative and positive regulation by a short segment in the 5'-flanking region of the human cytomegalovirus major immediate-early gene

Journal Article · · Mol. Cell. Biol.; (United States)
; ;

To analyze the significance of inducible DNase I-hypersensitive sites occurring in the 5'-flanking sequence of the major immediate-early gene of human cytomegalovirus (HCMV), various deleted portions of the HCMV immediate-early promoter regulatory region were attached to the chloramphenicol acetyltransferase (CAT) gene and assayed for activity in transiently transfected undifferentiated and differentiated human teratocarcinoma cells, Tera-2. Assays of progressive deletions in the promoter regulatory region indicated that removal of a 395-base-pair portion of this element (nucleotides -750 to -1145) containing two inducible DNase I sites which correlate with gene expression resulted in a 7.5-fold increase in CAT activity in undifferentiated cells. However, in permissive differentiated Tera-2, human foreskin fibroblast, and HeLa cells, removal of this regulatory region resulted in decreased activity. In addition, attachment of this HCMV upstream element to a homologous or heterologous promoter increased activity three-to fivefold in permissive cells. Therefore, a cis regulatory element exists 5' to the enhancer of the major immediate-early gene of HCMV. This element negatively modulates expression in nonpermissive cells but positively influences expression in permissive cells.

Research Organization:
Dept. of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037 (US)
OSTI ID:
7154648
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 7:11; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
550400* -- Genetics
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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CELL DIFFERENTIATION
CHLORAMPHENICOL
CONNECTIVE TISSUE CELLS
DATA
DNA-ASE
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERASES
EXPERIMENTAL DATA
FIBROBLASTS
GENE REGULATION
HELA CELLS
HOST-CELL REACTIVATION
HYDROLASES
INFORMATION
MAMMALS
MAN
MICROORGANISMS
NUMERICAL DATA
PARASITES
PHENOTYPE
PHOSPHODIESTERASES
PRIMATES
RECOVERY
REPAIR
SOMATIC CELLS
TRANSFERASES
TUMOR CELLS
VERTEBRATES
VIRUSES