Inducible binding of a factor to the c-fos regulatory region
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
The c-fos gene is rapidly and transiently activated in quiescent BALB/c-3T3 cells in response to serum, platelet-derived growth factor or conditioned medium from v-sis-transformed cells. This activation occurs at the level of transcription and in the absence of new protein synthesis. Using a gel electrophoresis DNA-binding assay, they have found a DNA-binding activity in BALB/c-3T3 cells that is induced within 20 min of treatment with conditioned medium from v-sis-transformed cells. A DNA methylation interference assay has shown that this factor binds to a sequence approximately 346 base pairs upstream of the transcription initiation site of the human c-fos gene. Insulin, epidermal growth factor, and phorbol 12-myristate 13-acetate fail to induce this DNA-binding factor. Protein synthesis inhibitors do not block the induction of this activity. The authors propose that this factor preexists in an inactive form in quiescent cells and that its binding activity is response is appropriate extracellular inducers.
- Research Organization:
- Massachusetts Institute of Technology, Cambridge
- OSTI ID:
- 6125544
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:5; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BLOOD SERUM
CARCINOGENS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
ELECTROPHORESIS
ESTERS
FIBROBLASTS
GENE REGULATION
GENES
GROWTH
HORMONES
INSULIN
ISOTOPES
LIGHT NUCLEI
MAMMALS
METHYLATION
MICE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TRANSCRIPTION
VERTEBRATES
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BLOOD SERUM
CARCINOGENS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
ELECTROPHORESIS
ESTERS
FIBROBLASTS
GENE REGULATION
GENES
GROWTH
HORMONES
INSULIN
ISOTOPES
LIGHT NUCLEI
MAMMALS
METHYLATION
MICE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TRANSCRIPTION
VERTEBRATES